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Fig. 10. Defects in laminin 5 deposition, prevent adhesion and dephosphorylation of P-p38. MKs from laminin 5 null mice (
3-/-) and wild-type mice (3+/+) were suspended and replated onto either a BSA- or Laminin 5-coated surface. Triton-soluble extracts were collected at 2 hour intervals from 0 to 10 hours and immunoblotted with anti-P-p38 and anti-p38 antibodies. Quiescent 3+/+ and 3-/- MKs did not express phosphorylated p38. When plated onto a BSA-coated surface, 3+/+ MKs deposited laminin 5, adhered to the deposits on the BSA surface and dephosphorylated p38 within 4 hours. In contrast, 3-/- MKs were unable to deposit laminin 5 or adhere to the BSA or dephosphorylate p38 for the duration of the assay. Both 3+/+ MKs and 3-/- MKs rapidly adhered to exogenous laminin 5 and dephosphorylated P-p38, indicating exogenous laminin 5 was sufficient to rescue the ability of 3-/- MKs to adhere and dephosphorylate p38. Total levels of p38 did not change significantly in either the 3+/+ MK or 3-/- MK populations.
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