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Fig. 6 (A) P-p38 and P-ATF2 are elevated in LKs (white arrowheads) of scrape wounds. Scrape wounds were generated in confluent HK monolayers. Levels of phosphorylated p38 and ATF2 were examined 1 hour post wounding by immunofluorescence microscopy. (B). P-p38 is upregulated in LKs of wounds in vivo. Cryostat sections of human epidermis 0 hours (a), 1 hour (b) and 4 hours (c) post wounding were examined for p38 phosphorylation through immunofluorescence microscopy. In the control (d), a 0 hour wound section is stained with anti-laminin 5 mAb (C2-5) to identify the basement membrane. Wound margins are identified with white arrowheads. Direction of outgrowth is shown by white arrow. Bar, 20 µm.
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