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Fig. 2. Analysis of the vascularization of CAR-deficent embryos. Wild type (+/+) and CAR-deficient (–/–) embryos of stage E10.5 (top) or E11.5 (middle) were stained as whole mounts with anti-PECAM-1 antibodies. The head region is shown for E10.5, limb buds are shown for E11.5. No obvious differences in overall vascularization were observed. Bottom panel: paraffin sections of E11.5 embryos. Sections were taken from the posterior limb region, and stained with hematoxylin and eosin (H+E). Note: aortae and cardinal veins were enlarged in CAR^-/- embryos. Key: a, left and right aorta; c, left and right cardinal vein. Bar, 200 µm. In each case, CAR^+/+ and CAR^-/- littermates were compared.

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