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Fig. 5. Abnormalities of the heart of CAR-deficent embryos. (A) Whole mounts of wild type (+/+) and CAR-deficient (–/–) E11.5 embryos of the same litter are shown. The asterisk marks the enlarged pericard in the CAR^-/- embryo. Hemorrhages resulting from preparing the embryos were more often observed in CAR^-/- embryos than in wild-type embryos. (B) Paraffin sections of E11.5 embryos of the same litter were counterstained with hematoxylin. The micrographs show the heart region. CAR^-/- embryos showed enlarged endocardial cushions and a single persistent atrioventricular canal instead of two canals in the wild-type embryo. Key: a, atrium; v, ventricle; e, endocardial cushion; ^*, atrioventricular canal. Bar, 100 µm.

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