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Fig. 8. Comparison of in vitro cultured wild type and CAR^-/- embryonal cardiomyocytes. Cardiomyocytes of E11.5 embryos isolated and cultured for 3 days were stained by indirect immunofluorescence with antibodies against sarcomeric ${alpha}$ -actinin. Three examples of typical wild-type cells (+/+) and CAR-deficient cells (–/–) are depicted. Note: the myofibrils were disorganized and shortened in CAR^-/- cells.

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