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Fig. 9. Orientation and localization of myofibrils in the vicinity of cell contacts and focal contacts in embryonal CAR+/+ and CAR-/- cardiomyocytes. (A) Indirect immunofluorescence of CAR+/+ cells (+/+) for CAR and of CAR+/+ cells and CAR-/- cells (–/–) for ZO-1. (B-D) Double staining of CAR+/+ and CAR-/- cells for: (B) N-cadherin (green) and ß-catenin (red); (C) ß1-integrin (green) and {alpha}-actinin (red); (D) N-cadherin (green) and {alpha}-actinin (red). In B and C the single stainings and the merge are shown (as indicated) whereas in D only the merged picture is depicted. Bars in A-C represent 50 µm, bar in D is 10 µm.





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