QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 1. ${alpha}$ -Syn overexpression inhibits TH Ser40 phosphorylation. Representative immunoblots from a single experiments comparing 20 µg total protein from MN9D and PC12 cells. (Left column) (A) ${alpha}$ -Syn expression is low in UT (lane 1) and plasmid-transfected MN9D cells (lane 2), but increased >10-fold in stably transfected ${alpha}$ -Syn MN9D cells (lane 3). (B) Total TH protein is abundant in all MN9D cells, although, in this experiment, a reduction in total TH is apparent in the ${alpha}$ -Syn-overexpressing cells, although that was not always noted for ${alpha}$ -Syn MN9D cells. (C), P-Ser40 levels are virtually identical for UT (lane 1) and plasmid transfected cells (lane 2), but significantly reduced in the ${alpha}$ -Syn MN9D cells (lane 3). (Right column) Similar to MN9D cells (A) ${alpha}$ -Syn expression is low in UT (lane 1) and plasmid-transfected PC12 cells (lane 2), but increased $~$ 20-fold in stably transfected ${alpha}$ -Syn PC12 cells (lane 3) at 72 hours after induction. (B) Total TH protein is equally abundant in all PC12 cells regardless of ${alpha}$ -Syn levels. (C) P-Ser40 levels are virtually identical for control PC12 cells (UT, lane 1; plasmid transfected PC12 cells, lane 2) but significantly reduced in the ${alpha}$ -Syn Pc12 cells (lane 3). (D) P-Ser40 data from several experiments were normalized to total TH and expressed as percentage relative to control UT MN9D (left) and PC12 (right) cells. Untransfected cells (UT), plasmid-transfected control cells (plasmid), and stably transfected ${alpha}$ -Syn-overexpressing cells ( ${alpha}$ -Syn). Values are mean ± s.e.m. from three independent experiments for each cell line. ^***P<0.001.

Return to article