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Fig. 5. Blockade of PP2A activity with okadaic acid demonstrates a role for PP2A in Ser40 dephosphorylation in MN9D and PC12 cells. PP2A inhibition by 10 nM-1.0 µM okadaic acid for 1 hour, caused an increase in P-Ser40 levels in all conditions when compared to parallel vehicle-treated controls for each condition. The baseline value was set to zero to demonstrate the fold increase in P-Ser40 levels above baseline for each condition. (A) Okadaic acid at low doses (0-100 nM) produced small changes in P-Ser40 in plasmid control MN9D cells but large significant increases in P-Ser40 in -Syn-overexpressing MN9D cells. Vehicle-treated cells were essentially unchanged from baseline at these concentrations of okadaic acid. (B) With higher dose okadaic acid, even more robust increases in P-Ser40 were noted for -Syn MN9D and induced -Syn PC12 cells, suggesting that low baseline P-Ser40 phosphorylation levels in -Syn overexpressors had probably occurred through effects on PP2A activation. Values are mean ± s.e.m. of two to six independent experiments. ^*P<0.01.

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