QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online August 16, 2005

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?

A two-armed spindle checkpoint

The spindle checkpoint maintains genomic stability by ensuring that mitosis cannot proceed until all chromosomes are correctly aligned on the spindle. Several kinases, including Bub1, BubR1 and aurora B, are implicated in spindle checkpoint function but exactly how anaphase onset is regulated remains unclear. On p. 3639, Stephen Taylor and co-workers reveal new details of the spindle checkpoint in human somatic cells by showing that Bub1 and aurora B cooperate to maintain BubR1-mediated inhibition of the anaphase promoting complex/cyclosome (APC/C), which targets anaphase inhibitors for degradation. The authors use time-lapse microscopy, RNAi-mediated knockdown of Bub1, chemical inhibition of aurora B, and microtubule-disrupting agents to build a new model for the spindle checkpoint. In this, the checkpoint has two arms: the Bub1 arm monitors attachment of microtubules to kinetochores (the proteinaceous structures that link chromosomes to the spindle) whereas the aurora B arm monitors bi-orientation (attachment of kinetochore pairs to spindles from opposite poles). The arms converge on the mitotic checkpoint complex, which includes BubR1, to promote its binding to and inhibition of APC/C, thereby delaying the metaphase-to-anaphase transition until all the chromosomes are correctly aligned on the spindle.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Related articles in JCS:

Bub1 and aurora B cooperate to maintain BubR1-mediated inhibition of APC/C^Cdc20

Christopher J. Morrow, Anthony Tighe, Victoria L. Johnson, Maria I.F. Scott, Claire Ditchfield, and Stephen S. Taylor
JCS 2005 118: 3639-3652. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?