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Fig. 9. CaM translocation is blocked by a nonphosphorylatable MARCKS ED decoy peptide. (A) Confocal images of distribution of fluorescent ED4A or ED4Ax in freshly dissociated portal-vein smooth-muscle cells (left) and quantitation of average surface:core fluorescence ratios from 9-11 cells (right). Arrowheads denote the position of the nucleus. Where no arrowhead is shown, the nucleus is beyond the field of view of the image shown. (B, left) Confocal images of cells fixed and immunostained to locate CaM after loading with ED4A or ED4AX either at rest or after stimulation with the phorbol ester DPBA. (B, right) Average surface:core fluorescence ratios from 9-11 cells. Arrowheads denote the positions of the nuclei. Where no arrowhead is shown, the nucleus is beyond the field of view of the image shown. (C) Average densitometry of immunoblots for Ser159/163 MARCKS phosphorylation for unstimulated tissues or tissues stimulated with DPBA and mock loaded or loaded with ED4A or ED4AX (n=4). *, P<0.05 compared with the mock-loaded control; #, P<0.05 compared with ED4AX.





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