QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 1. The absence of inhibitory Cdk1 tyrosine phosphorylation causes severe morphological defects in U. maydis. (A) Cdk1 is phosphorylated at Tyr¹⁵. Protein extracts from wild-type and TAU17 cells expressing a FLAG-tagged version of Cdk1 (cdk1-1) were subjected to immunoblotting with anti-phospho-Cdc2 (Tyr15) and anti-PSTAIRE antibodies. Cdk2 is the U. maydis homolog of S. cerevisiae Pho85, which is also recognized by the anti-PSTAIRE antibody. (B) Expression of the ectopic myc-tagged Cdk1. Protein extracts from wild-type, and UMC5 and UMC6 cells expressing myc-tagged Cdk1 (P_tef1:cdk1-myc) and the constitutively unphosphorylated Cdk1 allele (P_tef1:cdk1^AF-myc), respectively, were immunoblotted with anti-PSTAIRE antibodies. (C) UMC5 and UMC6 cells were grown to mid-exponential phase in rich medium (YPD) and then stained with DAPI and FITC-WGA, to visualize nuclei and cell walls, respectively. Scale bars: 20 µm.

Return to article