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Fig. 10. Impaired inhibitory phosphorylation of Cdk1 affects the ability of U. maydis to produce a successful plant infection. (A) Disease symptoms on the leaf blades of young maize plants 14 days post-inoculation with the strains indicated. Control infection (a1 b1 P_mig1:cdk1 x a2 b2) produced the characteristic symptoms of disease (observe the red stain produced by the anthocyanin streaking and small tumors at the leaf margin). In contrast, infection with cells expressing the cdk1 allele refractory to Tyr¹⁵ phosphorylation (a1 b1 P_mig1:cdk1^AF x a2 b2) did not induce symptoms other than chlorosis (observe the yellowing of the leaf tissue). (B) Hyphal development of a1 b1 P_mig1:cdk1 x a2 b2 (upper panel) and a1 b1 P_mig1:cdk1^AF x a2 b2 (bottom panel) cells inside the plant tissue. Symptomatic leaves were removed one week after inoculation and the presence of fungal cells was detected by staining with Chlorazole Black E. Observe the dramatic difference in growth between the a1 b1 P_mig1:cdk1^AF x a2 b2 hyphae (bottom panel) and the controls, where two branching hyphae can clearly be seen (upper panel). Scale bars: 20 µm.

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