spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Cdk1 phosphorylation and S phase checkpoint. (A) Effects of inhibition of DNA synthesis by hydroxyurea on Cdk1 phosphorylation. HU (1 mg/ml) was added to an early log phase culture of FB1 cells and samples were taken at 30 and 60 minutes after HU addition. (B) Impaired tyrosine phosphorylation of Cdk1 causes sensitivity to HU. Wild-type (FB1), and UMC5 and UMC6 cells expressing myc-tagged Cdk1 (Ptef1:cdk1-myc) and the constitutively unphosphorylated Cdk1 allele (Ptef1:cdk1AF-myc), respectively, were spotted as serial dilutions onto YPD plates containing a series of HU concentrations and the plates were incubated at 28°C for 3 days.





Right arrow Return to article