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Fig. 8. Cells in which wee1 is transcriptionally deregulated, do not adapt to different nutritional conditions. (A) The levels of wee1 mRNA are transcriptionally regulated by nutritional conditions. Wild-type FB1 cells (WT) and the mutant UMC44 strain (wee1^scp) in which the endogenous wee1 promoter has been exchanged for the scp promoter, were grown in nutrient-rich (YPD), complete (CMD) and minimal medium (MMNO₃) to OD₆₀₀ 0.5. Their RNA was isolated and 10 µ g of RNA was loaded per lane, using 18s rRNA as a loading control. (B) Length of wild-type and wee1^scp cells growing in different media. The length of the major axis of 120 cells was measured and plotted as a function of the number of cells. (C) DNA content of wild-type and wee1^scp cells growing in different media.

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