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Fig. 1. Immunolocalization of moesin in CD8 cells. (A) Cells were either left untreated (CTR) or stimulated with forskolin (FK). After fixation, cells were immunostained with anti-moesin antibody (1:100 dilution). Fluorescence was visualized by epifluorescence microscopy and the xz reconstructions were obtained by deconvolution using Autodeblur software (insets). In untreated cells, moesin was predominantly localized at cell adhesion sites. In FK-stimulated cells, moesin was mainly expressed at cell-surface structures resembling apical microvilli. Bar, 5 µm. (B) Moesin distribution in subcellular fractions. Equal amounts of protein (30 µg/lane) from particulate (P, 150,000 g pellet) and soluble (S, 150,000 g supernatant) fractions from control and forskolin-stimulated cells were separated by gel electrophoresis and immunoblotted with anti-moesin antibody. Equal loading was confirmed by Coomassie blue staining. On the right, the densitometric analysis of the revealed bands is shown (*P<0.01, #P<0.001, with respect to control; n=3).
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