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Fig. 1. Constitutive and induced expression of cellular hsps. Non-stressed B-cell lines were washed in PBS, fixed, permeabilised and stained with hsp-specific antibodies or isotype control (as indicated). Levels of hsps were examined by flow-cytometry. (a) The value for the mean fluorescence intensity (MFI) was taken (mean+s.e.m. of four B-cell lines). (b) Altered cellular hsp expression following heat stress. Four B-cell lines were incubated at 42°C for up to 3 hours, incubated for a total of 24 hours at 37°C before hsp expression was analysed by flow-cytometry. The graph is expressed in terms of fold-change relative to non-stressed expression (normalised to a value of 1, and indicated by the dotted line) (mean ± of four B-cell lines).
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