spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. RNAi reduces Bub1 levels to ~2% in ~70% of cells. HeLa and DLD-1 cells were transfected with either control or Bub1 siRNA duplexes then analyzed 48 hours later. (A) Blot of HeLa cell lysates showing that, across a population, RNAi reduces Bub1 levels to 10-25%. In order to quantitate the level of repression, the lysate from the Bub1 RNAi culture was analyzed side by side with a lysate from a control RNAi culture diluted in lysis buffer to 50, 25 and 10% of its original concentration. (B) Quantitation of immunofluorescence analysis in DLD-1 cells demonstrating that while ~70% of the mitotic cells in Bub1-RNAi cultures show low levels of Bub1 expression, ~18% appear untransfected and ~12% appear partially repressed.





Right arrow Return to article