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Fig. 2. Bub1-RNAi cells enter anaphase with unaligned chromosomes. DLD-1 cells expressing a GFP-histone fusion protein were transfected with siRNA duplexes designed to target (A) lamin B1, (B) BubR1 or (C) Bub1. The panels represent images taken from time-lapse sequences with the time shown in minutes. Bars, 5 µm. Note that the cell in panel C was already in prometaphase at the start of the time-lapse sequence, so the values underestimate the time spent in mitosis.





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