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Fig. 1. IGF-1 stimulates the phosphorylation and SHP-2 binding of IRS-2 in growth cones. GCPs were stimulated with IGF-1 or incubated in control conditions for 1 or 5 minutes. IRS-2 was immunoprecipitated, and blots of the precipitates were probed with antibodies to pTyr, SHP-2 and IRS-2 as a loading control.





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