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Fig. 2. IGF-1 increases phosphorylation of the PI3K subunit p85 in growth cones. Phosphotyrosine (PY20) immunoprecipitates were prepared from GCPs incubated in control medium or with 1 nM IGF-1 for 5 minutes. Blots were probed with anti-p85 antibody. IP, immunoprecipitate; SN, supernatant polypeptides after immunoprecipitation. A substantial increment of p85 is evident in the immunoprecipitate of GCPs stimulated with IGF-1. Relative optical densities of p85 are shown at the bottom of the figure (arbitrary units; means±s.e.m. of three independent experiments).





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