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Fig. 3. IGF-1 enhances Akt phosphorylation in growth cones. GCPs were incubated in control conditions or stimulated with IGF-1 for 30 seconds, 1 minute or 5 minutes. Western blots of polypeptides were probed with anti-p85 and with a phosphorylation-specific antibody to Akt (AktS473). p85 served as a loading control. The relative O.D. (A.U.) of AktS473 normalized over p85 signal are shown at the bottom of the figure. Those results are the means±s.e.m. of three (30 seconds and 5 minutes) or two (1 minute) independent experiments.





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