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Fig. 2. The same numbers of Langerhans cells (Langerin/CD207+) but different collagen content characterize the ear skin of SPARC+/+ and SPARC–/– mice. (A) The distribution of Langerin/CD207+ cells in the epidermal sheet from untreated SPARC+/+ and SPARC–/– mice is very similar. Cell counting and morphometry revealed essentially the same numbers of LCs in the epidermal sheet of the two strains (SPARC+/+=50.9±7.5 and SPARC–/–=53.8±4.4 in 10 randomly chosen fields, n=5). (B) Total ear collagen content and distribution are shown in cross-sections of the middle auricles by trichromic stain. SPARC+/+ ear pinna is characterized by a greater amount of collagen on both sides of the cartilage; increased thickness of the internal face of auricles and minor evidence of external auricle subcutaneous fat and muscle structures are seen compared with SPARC–/– ear pinnae.





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