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Fig. 3. Distribution of Langerhans/CD207+ cells into the draining LN of SPARC+/+ and SPARC/ mice after DNFB sensitization. Draining LNs were removed 24 and 48 hours after sensitization, sectioned and stained with mAb to Langerin/CD207. Langerin/CD207+ cells were counted under a microscope with a 20x objective. Panel A shows representative Langerin staining of draining LN from SPARC+/+ and SPARC/ mice collected 24 and 48 hours after DNFB painting. Panel B shows the number (mean±s.d.) of Langerin/CD207+ cells counted in 10 randomly chosen fields from six independent preparations for each SPARC+/+ and SPARC/ experimental group.