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Fig. 4. Migration of LX-bearing cells in the draining LN and consequent increase in LN cellularity. (A) SPARC^+/+ and SPARC^–/– were injected with 10⁷ green-LX-microspheres in the dorsal side of the right ear, which was painted with DNFB 30 minutes later. Draining LNs were collected after 8 and 24 hours and cells were immunolabeled with PE-conjugated anti-I-A^d/I-E^d for two-color FACS analysis. A higher number of green-LX-loaded/I-A^d/I-E^d+ cells were found in draining LNs from SPARC^–/– than from SPARC^+/+ mice at both 8 and 24 hours after treatment. (B) Morphology of green-LX-loaded/I-A^d/I-E^d+ cells in the draining LN reveals the typical hairy and veiled appearance of DCs. (C) Total cell number in the draining LN of treated mice. Migration of green-LX-loaded/I-A^d/I-E^d+ cells in the draining LN is associated with increased LN cellularity which is more extensive in SPARC^–/– mice.

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