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Fig. 5. Migration of BM DCs does not depend on their SPARC genotype. We pre-loaded in vitro BMDCs from SPARC^+/+ and SPARC^–/– mice with green- or red-LX microspheres, respectively (or vice versa), before co-injecting them (5x10⁵ each) intradermally into the ear dorsal side of SPARC^+/+ or SPARC^–/– mice. Draining LN were collected after 48 hours and analyzed by FACS. (A) An example of SPARC^+/+ green-LX- and SPARC^–/– red-LX-loaded DCs in draining LN from SPARC^+/+ mice. (B) Cumulative data of all tested combinations. A higher number of green- or red-LX-loaded DCs was found in draining LNs of SPARC^–/– mice, regardless of the SPARC genotype of the LX-loaded DCs. Each bar represents the mean value of 14 LNs analyzed (7 mice/group).

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