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Fig. 6. Effect of DC migration on T-cell priming. Panel A shows the accelerated CHS occurring in SPARC^–/– mice. DNFB-sensitized SPARC^+/+ and SPARC^–/– mice were challenged with DNFB starting 48 hours after sensitization and every other day until day 5. Ear swelling was measured 24 hours after challenge. At every time point, ear swelling was significantly higher (P<0.05) in SPARC^–/– than in SPARC^+/+ mice. Panel B shows DTH elicited in SPARC^–/– but not SPARC^+/+ challenged mice 48 hours after OVA sensitization. Panel C shows the effect of DC migration on priming of OVA-specific transgenic T cells. CFSE-labeled KJ⁺CD4⁺CD25^– cells were adoptively transferred into SPARC^+/+ and SPARC^–/– mice. Mice were immunized 8 hours later by injection of OVA protein into the ear pinna. Draining LNs were isolated at the indicated time points after immunization and stained with KJ-126-PE Ab. the histogram show the analysis from a representative mouse at each time point for SPARC^+/+ and SPARC^–/– groups. See Table 1 for quantitative data analysis. M1 and M2 indicate non-divided and divided cells, respectively.

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