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Fig. 3. Dominant-negative effect of C-terminally tagged ApsB-GFP on MT organization. (A) In wild type, MTs (GFP-TubA (a) connect adjacent nuclei [StuA(NLS)-DsRedT4 (b)] (SJW100) and several additional MTs emerge from the nuclear SPB [arrowheads in overlay (c)]. (B) In strains with N-terminally tagged ApsB, MTs were organized like in wild type (SDV1B). (b) mRFP1-ApsB dots indicate the position of the nuclear SPB of two nuclei along cytoplasmic MTs [GFP-TubA (a)] [dark areas and arrowheads in overlay (c)=nuclei]. (C) C-terminally tagged ApsB-GFP (SDM40) displayed a dominant negative phenotype reducing the number of MTs similar to the apsB10 mutation (D) (SDV16). In (C) notice the MT-bound ApsB-GFP dot (middle) and the nuclear SPB-associated ApsB-GFP [bright, white dots connecting two nuclei to MT (oval, white areas of StuA(NLS)-GFP]. Bar, 3 µm for (A and B) and 4 µm for (C and D).





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