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Fig. 6. Genetic interaction of apsA and apsB with molecular motors. (A) Strains were grown for three days at 37°C on glucose medium (a) to repress or on ethanol medium (b) to induce the alcA-promoter. A ${Delta}$ kinA strain (SNR3) grows more compact compared to wild type, but sporulation is not affected. Hyphal growth of a ${Delta}$ apsA strain (SRF30) is similar to wild type (RMS011), but spore production is reduced (brown colony). A double mutation of ${Delta}$ kinA and ${Delta}$ apsA (SDM24) results in a synthetic lethal phenotype, while strains, which contained an inducible alcA(p)::apsA::GFP construct in addition to the ${Delta}$ kinA and ${Delta}$ apsA mutation (SDM23), produces spores and shows normal growth on ethanol medium apart from the compact colony morphology due to the influence of the ${Delta}$ kinA mutation. The cortical localization of ApsA-GFP was not influenced by the ${Delta}$ kinA mutation (not shown). (B) ${Delta}$ nudA and ${Delta}$ nudF strains grow very poorly, but grow better when suppressed by the apsB14 mutation. In comparison, apsA5 has no effect on the dynein deletion strain. Strains were incubated at 43°C for 3 days. Strains from left to right are: apsB14/ ${Delta}$ A-7, apsB14/ ${Delta}$ A-9, apsB14/ ${Delta}$ F-9, apsB14/ ${Delta}$ F-11, apsA5/ ${Delta}$ A-6, XX60, apsB14/ ${Delta}$ F-3, apsB14/ ${Delta}$ F-6, SEwild-type and apsB14. Bars, 1 cm (A,B) and 1.5 cm (B).

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