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Fig. 7. JNK and Nox signal to Myc for the induction of DNA synthesis. (A) RacV12 rescues the Myc induction defect in Abl/Arg–/– cells. Indicated quiescent cells infected with wild-type (mock) or indicated retroviruses were stimulated or not with PDGF for 1 hour. The level of Myc mRNA was quantified by real-time quantitative RT-PCR as described in Materials and Methods. The ratio of Myc mRNA in stimulated and non-stimulated cells is shown (Myc induction). (B) Myc induction is sensitive to inhibitors of JNK and Nox activities. Quiescent NIH3T3 treated with DMSO (control) or indicated inhibitors (10 µM or 10 mM for NAC) were stimulated or not for 1 hour with PDGF. Myc mRNA was assessed by northern blotting as described in Materials and Methods. % Myc mRNA level=[Myc mRNA level]/[Myc RNA level of control quiescent cells]. Myc rescues the G1 block induced by DN-MKK4 (C) and p67V204A (D). Quiescent NIH3T3 transfected with indicated constructs were stimulated or not with PDGF in the presence of BrdU and proceeded as in Fig. 1B. The mean±s.d. of the percentage of BrdU-positive cells present in expressing and non-expressing cells under the specified conditions is shown.
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