JCS02510 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 (Adobe PDF) - Fig. S1. Sequence alignment of A, B, C subdomains of desmoplakin with PRDs of MACF1b.  The five conserved subdomains (A1, A2, C, iB, iA) of MACF1b were identified by comparing their sequence to the A, B, C subdomains of desmoplakin (DP-A, -B, -C). iB, incomplete B subdomain.  iA, incomplete A subdomain.

• Supplemental Figure 2 - Fig. S2. C1 and C2 subdomains of MACF1b does not target to Golgi apparatus in COS7 cells.  COS7 cells were transiently transfected with tagged cDNA constructs C1 (A and B), and C2 (D and E) for 18 hours, fixed and stained for HA tag (A and D; red) or Golgi (GM130; B and E; green).  C and F, superimposed images.  Annotation and location of HA tagged cDNAs are shown in Fig. 8A.

• Supplemental Figure 3 - Fig. S3. Knockdown of MACF1b does not affect the microtubule cytoskeleton in H460 cells.  H460 cells transfected with control vector (Control; A and B) or MACF siRNA vector (MACF-siRNA; D and E) were fixed and stained for b-tubulin (Tubulin; A and D) and GFP (B and E).  C and F, merged images.  Microtubule elements were not affected in siRNA-expressing cells, as revealed by co-expressed GFP.