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Fig. 8. Targeting of MACF1b-PRD to the Golgi complex in COS7 cells. (A) Illustration showing MACF1b PRD and cDNA clones that were used to generate HA-tagged MACF1b PRD constructs. FL, all PRD subdomains; N, two A sundomains; C1, C subdomains and the two incomplete B and A subdomains; C2, C-terminal region that did not include any PRDs. (B) Expression of HA-tagged MACF1b-PRD proteins in COS7 cells. COS7 cells that were transfected with various cDNA constructs (FL, N, C1, C2) were collected for western blot analysis with anti-HA antibody. Mk, mock-transfected cells. Bars on left indicate molecular mass standards: from top to bottom (kDa), 181.8, 115.5, 82.2, 64.2, 48.4 and 37.1. (C-H) Immunostaining of cells that were transfected with different cDNA clones of MACF1b-PRD. COS7 cells were transfected with cDNA constructs FL (C,D), N (E-H), and immunostained with antibodies against HA-tag (C,E,G) or GM130 (D,F,H). Bar, 20 µm.





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