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Fig. 9. Knockdown of MACF1b resulted in the disassembly and dispersion of the Golgi complex in H460 cells. H460 cells transfected with control vector (Control; A and B, E and F) or MACF-siRNA vector (MACF-siRNA; C and D, G and H) were fixed and stained for MACF1b (B,D), GM130 (F,H), and GFP (A,C,E,G). The siRNA plasmid contained a GFP cassette to identify the transfected cells. Note that MACF1b staining in cells treated with MACF1-siRNA (GFP positive) was greatly reduced (compare D with B) and the Golgi complex was disassembled (asterisks in D and H). Arrowhead in D indicates a cell that was not transfected with the MACF-siRNA vector (GFP negative) and displayed an undisrupted Golgi pattern. Bar, 20 µm.





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