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Fig. 2. Expression of myotilin reorganizes FATZ-1 in double-transfected CHO cells. CHO cells were transfected with HA-tagged FATZ-1 (A,C), with T7-tagged myotilin (B,D) or with both constructs (E-H). Expressed proteins were detected with the corresponding anti-tag antibodies. In single-transfected CHO cells, FATZ-1 has a periodical staining pattern along the stress fibers (see inset in C), whereas myotilin decorates the filaments evenly and bundles them (B,D and inset). In double-transfected cells, in which FATZ-1 (E,G) and myotilin (F,H) were coexpressed, both proteins show a high degree of colocalization and the periodical staining of FATZ-1 is lost. Bar, 5 µm.





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