QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 4. Yeast two-hybrid survey of the sites in myotilin mediating interaction with FATZ-1. The PJ69-4A yeast strain was co-transformed with pACT2 plasmids encoding various deletion mutants of myotilin in fusion with GAL4-activation domain (AD) and pAS2-1 plasmids encoding FATZ-1 fused at its N-terminus with GAL4-DNA-binding domain (BD). The N-terminal serine-rich domain and the two Ig-like domains of myotilin are represented by a dark grey box and the black loops, respectively. Growth was estimated after 5 to 10 days of incubation at 30°C. +, growth; –, no growth, on selective media. The results indicate an interaction between myotilin^1-498, myotilin^S55F or myotilin^T57I and FATZ-1, whereas shorter myotilin constructs did not interact. Transactivation controls were systematically performed for each construct with the opposite vectors without insert. Each experiment was repeated at least twice.

Return to article