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Fig. 5. Yeast two-hybrid survey of the sites in FATZ-1 mediating interaction with myotilin. Experiments were performed as described in Materials and Methods and Fig. 4. CD1 and CD2 regions of FATZ-1 are represented by dark grey boxes, whereas the glycine-rich domain is depicted as a white box. +, growth; –, no growth, on selective media. FATZ-175-299 and FATZ-1163-299 caused auto-transactivation (TA) on both selective media. *FATZ-1163-299 as GAL4-AD fusion protein was not transactivating. The results indicate an interaction between FATZ-11-299 or FATZ-1163-299 and myotilin.





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