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Fig. 7. The association frequency of the transgenic hygromycin phosphotransferase (HPT) locus with endogenous heterochromatin is similar in line A and in the mom1-1 background. Top row, Arabidopsis nuclei with intense DAPI-stained chromocenters. Middle row, the same nuclei after FISH with the 178 bp centromeric repeat (red) and pGL2 sequence labeling the HPT locus (green). From left to right: two, one or none of the separated allelic HPT signals associated with centromeric heterochromatin, and paired HPT signals associated or not associated with centromeric heterochromatin. Note the appearance of the HPT locus as small DAPI-positive chromocenter(s) in the second, third and fifth nucleus of the upper panel (arrows). Bottom row, the percentage of nuclei showing the corresponding type of association of HPT signals with centromeric heterochromatin (line A, n=269 nuclei; mom1-1, n=355 nuclei). Of the total HPT signals 30% were paired (see fourth and fifth nucleus) in line A and 21% in mom1-1. In nuclei of line A, 61% of the 271 HPT sites colocalizing with centromeric heterochromatin did so as single sites and 39% as paired sites. In mom1-1 nuclei, 65% of the 346 HTP sites colocalized with centromeric heterochromatin as single sites and 35% as paired sites.





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