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Fig. 1. Distribution of the four ubiquitously expressed mammalian SCAMPs in NRK cells. (A-D) low magnification wide-field images of SCAMP1-4 (SC1-SC4), emphasizing their concentration in perinuclear compartments. (E-G) Digitally deconvolved sections comparing the distribution of SCAMP1 to each of the other SCAMPs individually. SCAMP1 was detected using a biotinylated antibody 1 ${alpha}$ and SCAMP2 using 2 ${tau}$ , SCAMP3 using 3 ${gamma}$ and SCAMP4 using 4 ${omega}$ . Insets at lower left illustrate the extent of overlap (yellow) at perinuclear sites while the insets at lower right are representative views showing limited overlap of isoforms in the peripheral cytoplasm. Bar, 10 µm.

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