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Fig. 2. Distribution of SCAMP1-3 on transferrin-loaded endosomes as viewed by immunoelectron microscopy on A431 cell whole mounts. (A-C) Examples of specimens stained for all three SCAMPs using primary antibody-gold conjugates: SCAMP1 (SC1), 5 nm gold; SCAMP2 (SC2), 10 nm gold; and SCAMP3 (SC3), 15 nm gold. The electron-dense areas represent HRP stain within endosomes. Clusters of SCAMP1, 2 and 3 are circled and numbered accordingly. The arrow in C identifies the plasma membrane and enables visualization of the absence of extracellular background staining. (D) Control specimen stained with affinity-purified rabbit IgG-10 nm gold at the same concentration used in the other samples. Bar, 100 nm.





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