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Fig. 8. Distribution of GFP-tagged Rabs 5, 4, and 11 in relation to endogenous SCAMPs illustrated using SC1 and SC4 as examples. (A-F) deconvolved images of cells expressing wild-type rabs: rab5 (A,B), rab4 (C,D) and rab11 (E,F) and stained for SCAMP1 (A,C,E) and SCAMP4 (B,D,F). Insets show extent of rab-SCAMP overlap at higher magnification from marked peripheral (A-D) and perinuclear (E,F) cytoplasmic regions. Left insets in A,B illustrate enlarged circular endosomal profiles typically observed in cells expressing rab5-Q67L with focal peripheral SCAMP staining. (G-R) Whole cell images of cells expressing GFP-tagged dominant-negative rab5-S34N (G-J), rab4-I121N (K-N) and rab11-S25N (O-R) and comparing GFP staining (G,I,K,M,O,Q) to SCAMP1 (H,L,P) and SCAMP4 (J,N,R). Cells expressing GFP-rab are indicated by arrows. Bar, 10 µm.





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