JCS02515 Supplementary Material

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• Supplemental Figure 1 - Fig. S1. High frequency stimulation (HFS)-induced membrane currents and depolarisations in GluT and GluR cells. HFS (100 Hz) was applied for 1 second to the Schaffer collateral fibers. The stimulation period is indicated by grey boxes. (A) Analysis of a GluT cell (V_rest=–78 mV, R_i=5.2 MV, C_m=85 pF, A₁). The current pattern (A₁) was evoked according to the protocol given in Fig. 2. During HFS, membrane potentials or currents (lower traces) and extracellular field potentials (upper traces) were simultaneously recorded. (A₂) The GluT cell was depolarised by about 20 mV and repolarized mono-exponentially (t=6.21 seconds). (A₃) The corresponding membrane currents amounted to 587 pA and decayed with t=6.86 seconds (B) Analysis of a GluR cell (V_rest=–84 mV, R_i=89 MV, C_m=29 pF, B₁). (B₁) gives the current pattern of the cell. (B₂) HFS led to a depolarisation by 79.3 mV (from –83.9 to –4.5 mV). In contrast to the GluT cell, the membrane repolarized bi-exponentially (t₁=0.6 seconds, 77%; t₂=6.37 seconds, 23%). (B₃) In the voltage clamp mode (–80 mV), inward currents of 280 pA were induced (t₁=0.6 seconds, 69%; t₂=5.02 seconds, 31%). (C) For comparison, the HFS-induced whole cell responses of (A) and (B) were superimposed.

• Supplemental Figure 2 - Fig. S2. Variable current patterns of GluR cells receiving GABAergic synaptic input. Whole cell currents were evoked as described in the legend to Fig. 2A. All cells were weakly EGFP-positive and were located in the stratum radiatum of the CA1 subfield of p9 mice. All cells showed at least GABAergic synaptic input. (A) Typical innervated GluR cell (R_i=137 MV, V_rest=–85 mV, C_m=32 pF). (B) Characteristic components of the whole cell current pattern are given at higher resolution. (C) Whole cell current profiles of different GluR cells (scaling similar to A) displaying variable inward rectification and outward current amplitudes. The cell size did not correlate with the current pattern [from top to bottom R_in (MV): 84, 264, 575, 1435; V_rest (mV) –89, –79, –73, –68; C_m (pF): 35; 28; 48; 33].