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Fig. 2. LOS is required for the formation of actin-rich cell projections promoting bacterial uptake and for cortactin recruitment. HBMECs infected for 3 hours with the 2C43 wild-type strain of N. meningitidis (WT) or the isogenic rfaC-defective mutant strains ({Delta}rfaC) were double stained for actin (red) and ezrin (green) (A), for ErbB2 (green) and bacteria (red) (B) or for cortactin (green) and bacteria (red) (C), and were analysed by confocal microscopy. Merged images of the same fields are presented on the right-hand panels (overlay). (D) The frequency of formation of actin-rich cell projections or of bundle of actin filaments and of cortactin recruitment at the entry site of WT, {Delta}rfaC or {Delta}lgtA bacterial strains was determined by counting 50 cells. Average values (± s.e.m.) are presented from four independent experiments.





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