QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 5. LOS induces the activation of Rac1 GTPase and PI3K. HBMECs, transiently transfected with wild-type Rac1 coupled to GFP (GFP-Rac1), with the GTPase-binding domain of PAK1 coupled to GFP (GFP-PAK1-PBD) or with the PH domain of Akt coupled to GFP (GFP-Akt-PH) were infected for 3 hours with either the 2C43 wild type (WT) or the isogenic rfaC (rfaC) or lgtA (lgtA) defective mutants of N. meningitidis. When indicated (WT + wortmannin), HBMECs were pretreated for 2 hours with 100 ng ml^–1 wortmannin and then infected with the 2C43 wild-type strain of N. meningitidis in the presence of the inhibitor. (A-C) Cells were then stained for bacteria or ezrin (red) and recruitment of the different GFP-tagged proteins at bacterial entry sites was analysed by confocal microscopy. (right) Merged images (overlay) of the same fields. (D) The frequency of recruitment of the different GFP-tagged proteins at the entry site of WT, rfaC or lgtA bacterial strains was determined by counting 50 cells. Average values (± s.e.m.) are presented from three independent experiments.

Return to article