spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. Kinase-dead PCTAIRE-1 disrupts protein localization within the early secretory pathway. Cells were transfected with plasmids to express GFP-PCTAIRE-1 (wild type or point mutants as indicated). (A) The K184R ('kinase-dead') point mutation in PCTAIRE-1 results in fragmentation of the Golgi (giantin) and redistribution of ERGIC-53 to a more dispersed localization. In contrast, the wild type or `active' mutants of PCTAIRE-3 do not cause these effects. (B) The K184R point mutation in PCTAIRE-1 results in redistribution of the ER-Golgi SNARE protein membrin to a more dispersed localization (asterisk), and a reduction in the intensity and juxtanuclear clustering of COPII-coated ERES marked with Sec24Dp. In contrast, the wild type or `active' mutants of PCTAIRE-3 do not cause these effects.





Right arrow Return to article