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Fig. 5. CA-CamKII could overcome the inhibitory effects of KN-93 on eggs. (A) Experimental design. Eggs were treated with a parthenogenetic stimulus (Sr²⁺/PLC ${zeta}$ ) but were kept arrested at MetII by 10 µM KN-93 for a period of 2 hours. CA-CamKII cRNA was then microinjected into eggs to determine if it could overcome the inhibitory effects of KN-93. (B) Following microinjections of 3 pg CA-CamKII cRNA all eggs activated (30/30 Sr²⁺; 30/30 PLC ${zeta}$ ); inset shows decondensed chromatin stained with Hoechst. The vast majority of control eggs not microinjected with CA-CamKII cRNA remained arrested at MetII (rates of resumption of meiosis: 4/26 Sr²⁺; 0/25 PLC ${zeta}$ ): inset (right) shows the condensed chromatin of a MetII spindle stained with Hoechst. Scale bar: 20 µm.

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