|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 10. Ubiquitylation of endocytosed FGFRs. HeLa cells were cotransfected with myc-ubiquitin and FGFR1 (R1), FGFR4 (R4) or the empty pcDNA3 vector (-) and starved overnight. Cell-surface proteins were biotinylated and the cells were incubated for the indicated periods of time at 37°C in the presence of 200 ng/ml FGF1, 20 U/ml heparin and 0.3 mM leupeptin. After lysis, solubilised receptor proteins were adsorbed to streptavidin-Sepharose and analysed by immunoblotting (IB) with anti-myc antibody. The membrane was stripped and reprobed with anti-phospho-FGFR antibodies (anti-pFGFR) and anti-transferrin receptor antibodies (anti-TfR).
|
