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Fig. 2. Colocalization of FGFRs and endocytosed FGF1 after 2 hours. HeLa cells transiently transfected with FGFR1, 2, 3 or 4 were incubated with Cy3-FGF1, 50 U/ml heparin and 0.3 mM leupeptin for 2 hours at 37°C. The cells were then fixed, permeabilized and treated with rabbit anti-FGFR1, anti-FGFR2, anti-FGFR3 or anti-FGFR4 primary antibodies. The cells were further treated with Cy2-conjugated anti-rabbit secondary antibodies and examined by confocal microscopy. Arrows point to colocalization. Bar, 5 µm.
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