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Fig. 7. Degradation of the endocytosed 18 kDa form of FGF1. (A) HeLa cells, transiently transfected with FGFR1, 2, 3 or 4 were incubated with the [35S]methionine-labelled 18 kDa form of FGF1 and 20 U/ml heparin at 37°C for 1 hour to allow endocytosis to occur. The cells were then either lysed immediately (0 h) or incubated further in growth medium with or without chloroquine (chl) at 37°C for 3 or 6 hours before lysis. FGF1 was extracted from the lysate by binding to heparin-Sepharose and analysed by SDS-PAGE. (B) For each receptor the amount of 18 kDa form of FGF1 was calculated at each time point and expressed as a percentage of the amount of 18 kDa form at time zero. Values are averages of five independent experiments for FGFR1 and FGFR4 and of three independent experiments for FGFR2 and FGFR3. Error bars denote the s.d.
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