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Fig. 10. Effect of 4OHT on p120^ctn localization in LNER cells and association of p120^ctn and vimentin in L cells. (A) Immunofluorescent light microscopic localization of p120^ctn in LNER cells treated (+) or not (-) with 4OHT. (B) Immunoblot analysis of p120^ctn in immunoprecipitates from non-transfected parental L cells immunoprecipitated with anti-vimentin (Vm) or control (Cont) anti-myc antibody. Immunoblot analysis of L cell extract (Extract) indicates the position of p120^ctn. (C) Non-transfected parental L cells were processed for immunofluorescence using rabbit anti-p120^ctn (p120) and mouse anti-vimentin (Vm) followed by Cy3-labeled anti-rabbit IgG and FITC-labeled anti-mouse IgG. Arrows indicate regions of the cell where both p120^ctn and vimentin are abundant and colocalize. Bar, 50 µm.

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