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Fig. 4. Immunofluorescence light microscopic detection of NcadER following low concentration NP40 treatment of living LNER cells treated or not with 4OHT. Monolayer cultures of LNER cells previously treated or not with 100 nM 4OHT for 24 hours were exposed to 0.05% NP40 for 1 minute at room temperature prior to fixing and staining the cells with anti-N-cadherin. In the absence of 4OHT treatment much of the NcadER was removed by NP40 treatment of live cells (A). In contrast, the NcadER signal in LNER cells treated with 4OHT was significantly greater and was apparent at cell-cell borders (B). Bar, 50 µm.
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