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Fig. 6. Immunofluorescence light microscopic analysis of the effect of cytochalasin B on ß-catenin in LNER cells with or without 4OHT. LNER cells with or without 4OHT treatment were cultured in the presence of 1 µM cytochalasin B (Cyto B) to disrupt the actin cytoskeleton (see Fig. 5). The cells were fixed and stained with anti-ß-catenin to detect the NcadER/catenin complex. In LNER cells treated with Cyto B, but not 4OHT, the ß-catenin staining was weak and not particularly distinct at cell-cell borders (A). However, in cells treated with 4OHT and Cyto B, the ß-catenin staining was strong and distinct at cell-cell borders (B).
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